From:
sukiec@optonline.net
Date: 2006-10-01 21:49:31 UTC
Subject: [ferrethealth] Some recent abstracts and press releases
To: ferrethealth@smartgroups.com
=
Some recent abstracts and press releases
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START QUOTE
Lab Anim (NY). 2006 Oct;35(9):19.
Links
Exophthalmos and corneal edema in a young ferret.
Funk AJ, Rogers TD, Dobbins RM, Boyd K.
Department of Animal Resources, St. Jude Children's Research Hospital, 332 =
N. Lauderdale St., Memphis, TN 38105.
PMID: 17008903 [PubMed - in process]
END QUOTE
No abstract for it available at this point
---
Sorry, but this makes me cringe when gentler options such as using the exte=
rnal jugular are available:
START QUOTE
Lab Anim (NY). 2006 Oct;35(9):23-4.
Links
Blood collection from the cranial vena cava of the ferret.
Brown C.
The domestic ferret, though not as common a laboratory animal as the rat or=
mouse, serves as a model in critical research areas, including influenza b=
iology and vaccine development. Studies involving ferrets necessitate knowl=
edge of proper blood collection methods, such as cranial vena cava puncture=
.=
PMID: 17008905 [PubMed - in process]
Am J Respir Cell Mol Biol. 2006 Sep 28; [Epub ahead of print]
Links
Bioelectric Properties of Chloride Channels in Human, Pig, Ferret and Mouse=
Airway Epithelia.
END QUOTE
START QUOTE
Liu X, Luo M, Zhang L, Ding W, Yan Z, Engelhardt JF.
Departments of Anatomy and Cell Biology, University of Iowa, College of Med=
icine, Iowa City, Iowa, USA; The Center for Gene Therapy of Cystic Fibrosis=
and Other Genetic Diseases, University of Iowa, College of Medicine, Iowa =
City, Iowa, USA.
The development of effective therapies for cystic fibrosis (CF) requires an=
imal models that can appropriately reproduce the human disease phenotype. C=
F mouse models have demonstrated cAMP-inducible, non-CFTR chloride transpor=
t in conducting airway epithelia, and this property is thought to be respon=
sible for the lack of a spontaneous CF-like phenotype in the lung. Thus, an=
understanding of species diversity in airway epithelial electrolyte transp=
ort and CFTR function is critical to developing better models for CF. Two s=
pecies currently being used in attempts to develop better animal models of =
CF include the pig and ferret. In the study reported here, we sought to com=
paratively characterize the bioelectric properties of in vitro polarized ai=
rway epithelia -from human, mouse, pig and ferret- grown at the air-liquid =
interface (ALI). Bioelectric properties analyzed include amiloride-sensitiv=
e Na(+) transport, DIDS-sensitive Cl(-) transport, and cAMP-sensitive Cl(-)=
transport. Additionally, as an index for CFTR functional conservation, we =
evaluated the ability of four CFTR inhibitors, including glibenclamide, NPP=
B, CFTR inh-172 and CFTRinh-GlyH101, to block cAMP-mediated Cl(-) transport=
. In comparison to human epithelia, pig epithelia demonstrated enhanced ami=
loride-sensitive Na(+) transport. In contrast, ferret epithelia exhibited s=
ignificantly reduced DIDS-sensitive Cl(-) transport. Interestingly, althoug=
h the four CFTR inhibitors effectively blocked cAMP -mediated Cl(-) secreti=
on in human airway epithelia, each species tested demonstrated unique diffe=
rences in its responsiveness to these inhibitors. These findings suggest th=
e existence of substantial species-specific differences at the level of the=
biology of airway epithelial electrolyte transport, and potentially also i=
n terms of CFTR structure/function.
PMID: 17008635 [PubMed - as supplied by publisher]
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I do not know which of the parasites of these ermine may also set up shop i=
n ferrets:
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Wiad Parazytol. 2006;52(1):49-53.
Links
Parasites of carnivorous mammals in Bialowieza Primeval Forest.
Gorski P, Zalewski A, Lakomy M.
Department of Parasitology and Invasiology, Faculty of Veterinary Medicine,=
Warsaw Agricultural University, Poland.
BACKGROUND: Although the parasitofauna of wild carnivorous mammals in Polan=
d is quite well recognized, there has been only one research carried on thi=
s issue so far in Bialowieza Forest--the last lowland primeval forest of te=
mperate zone in Europe. MATERIAL AND METHODS: Twelve wild and two domestic =
species of carnivorous mammals are living in Bialowieza Forest. In our work=
faeces or intestines of all of them except ermine (Mustela erminea) have b=
een examined and parasites or their eggs (or oocysts) recorded. In total 22=
2 faecal probes from 13 species and 11 intestines of weasels have been inve=
stigated. RESULTS: All species of examined carnivorous mammals were infecte=
d with parasites. The most infected species has occurred red fox Vulpes vul=
pes (over 70% infected with parasites) meanwhile only about 30% of otters L=
utra lutra were infected. We found at least three protozoan species, one tr=
ematode, one tapeworm and nine species of nematodes. Except trematode Alari=
a alata, all of them are reported for the first time from Bialowieza Primev=
al Forest. Oocysts of coccidia have been found in faeces of nine host speci=
es, with the highest prevalence (29.4%) in badger. Six species of carnivoro=
us mammals have been occurred infected with trematodes (highest prevalence =
42.1% in wolves) and two with tapeworms (Diphyllobothrium latum with the pr=
evalence 2.6% in otter and 31.6% in wolves). All examined host species were=
infected with nematodes (with prevalence from 14.7% in lynx to 72.7% in re=
d fox). Results of our work should be treated as preliminary researches on =
the species composition of parasitic fauna invading carnivorous mammals in =
Bialowieza Primeval Forest.
PMID: 17007336 [PubMed - in process]
END QUOTE
Another influenza study:
<http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=3Dpubmed&cmd=3DRetrieve&d=
opt=3DAbstractPlus&list_uids=3D16991078&query_hl=3D4&itool=3Dpubmed_docsum>=
I think that I already carried this abstract on hearing, but in case I did =
not:
<http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=3Dpubmed&cmd=3DRetrieve&d=
opt=3DAbstractPlus&list_uids=3D16982164&query_hl=3D4&itool=3Dpubmed_docsum>=
Salmonella advance:
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Contact: Bill Cannon
cannon@pnl.gov
509-375-3732
DOE/Pacific Northwest National Laboratory
An infectious agent of deception, exposed through proteomics
The most detailed inventory yet of Salmonella proteins teases out how bacte=
ria invade immune cells while evading detection -- and presents a promising=
target for new drugs, vaccines and rapid diagnostics
RICHLAND, Wash. -- Salmonella bacteria, infamous for food poisoning that ki=
lls hundreds of thousands worldwide, infect by stealth. They slip unnoticed=
into and multiply inside macrophages, the very immune system cells the bod=
y relies on to seek and destroy invading microbes.
Just how Salmonella escapes detection by macrophages, turning predator cell=
s to prey complicit in promoting infection, has seemed impossibly complicat=
ed, a needle-in-a-haystack proposition involving thousands of proteins, the=
building blocks that carry out cells' vital functions.
Applying the high-volume sorting and analytical power of proteomics--a deta=
iled survey of microbial proteins present in the 24 hours that follow mouse=
-macrophage infection--a team led by Liang Shi of the Department of Energy'=
s Pacific Northwest National Laboratory has turned up a suspect protein.
The discovery of the protein, dubbed STM3117, is detailed today (Sept. 29) =
in The Journal of Biological Chemistry. Knocking out the gene that codes fo=
r STM3117, the researchers subsequently crippled the microbe's ability to m=
ultiply inside macrophages. Shi and colleagues say the protein and two clos=
ely related proteins discovered in the study are similar in genetic sequenc=
e to those known to make and modify chemicals in the microbe's cell wall ca=
lled peptidoglycan.
Drug and vaccine designers armed with this mouse-model information can targ=
et chemicals or immune responses that disrupt peptidoglycan synthesis and o=
ther processes linked to Salmonella's colonization of macrophages in humans=
, said Joshua Adkins, a co-author on Shi's paper and lead author of a relat=
ed study in Molecular & Cellular Proteomics last month. A quick identificat=
ion of these proteins, Adkins added, could help physicians assess the virul=
ence of a given strain.
The candidate proteins were winnowed from among 315 possibilities that emer=
ged through a combination of techniques, culminating in measurements by Fou=
rier-transform mass spectrometry, or FT-MS. A suite of FT-MS instruments cu=
stomized by co-author and PNNL-based Battelle Fellow Richard D. Smith enabl=
ed the team to rapidly separate and identify many proteins at once even as =
macrophages were being infected.
Most of the initial candidates were designated "house-keeping" proteins, or=
those whose numbers relative to other proteins remained more or less const=
ant during the course of infection. But 39 proteins shot up in number durin=
g bacterial colonization of macrophages, and of those, a handful or so--inc=
luding STM3117--responded specifically to a macrophage protein associated w=
ith resistance to microbial infection. A standard assay called Western blot=
confirmed the abundance increases of that small group of proteins during i=
nfection.
###
The work was funded by PNNL and the National Institutes of Health's Nationa=
l Institute of Allergy and Infectious Diseases, and much of the work was pe=
rformed at the PNNL-based W.R. Wiley Environmental Molecular Sciences Labor=
atory.
PNNL is a DOE Office of Science laboratory that solves complex problems in =
energy, national security, the environment and life sciences by advancing t=
he understanding of physics, chemistry, biology and computation. PNNL emplo=
ys 4,200 staff, has a $725 million annual budget, and has been managed by O=
hio-based Battelle since the lab's inception in 1965.
END QUOTE
-- Sukie (not a vet, and not speaking for any of the below in my private po=
sts)
Recommended health resources to help ferrets and the people who love them:
Ferret Health List
http://www.smartgroups.com/groups/ferrethealth
FHL Archives
http://ferrethealth.org/archive/
AFIP Ferret Pathology
http://www.afip.org/ferrets/index.html
Miamiferrets
http://www.miamiferret.org/fhc/
International Ferret Congress Critical References
http://www.ferretcongress.org
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