From:
Sukie Crandall
Date: 2007-06-02 05:37:46 UTC
Subject: [ferrethealth] E. coli infection: new research into inhibiting the formation of Shiga-toxin
To: ferrethealth@yahoogroups.com
Because the deaths of ferrets from E. coli has been recently=20
discussed here I though that this could be of interest:
http://www.pnas.org/cgi/content/abstract/0702589104v1
> Published online before print May 30, 2007
> Proc. Natl. Acad. Sci. USA, 10.1073/pnas.0702589104
>
> Microbiology
> Nitric oxide inhibits Shiga-toxin synthesis by enterohemorrhagic=20
> Escherichia coli
>
> ( bacterial infection | mucosal immunology )
>
> Marjolaine Vareille, Thibaut de Sablet, Thomas Hindr=E9, Christine=20
> Martin, and Alain P. Gobert *
> Institut National de la Recherche Agronomique (INRA), UR454 Unit=E9=20
> de Microbiologie, Centre de Recherches de Theix, 63122 Saint-Gen=E8s-
> Champanelle, France
>
> Edited by Roy Curtiss, Arizona State University, Tempe, AZ, and=20
> approved May 3, 2007 (received for review March 20, 2007)
> Shiga-toxin (Stx) is the cardinal virulence factor of=20
> enterohemorrhagic Escherichia coli (EHEC). The genes encoding Stx=20
> are carried by a lambdoid phage integrated in the bacterial genome=20
> and are fully expressed after a bacterial SOS response induced by=20
> DNA-damaging agents. Because nitric oxide (NO) is an essential=20
> mediator of the innate immune response of infected colonic mucosa,=20
> we aimed to determine its role in Stx production by EHEC. Here we=20
> demonstrate that chemical or cellular sources of NO inhibit=20
> spontaneous and mitomycin C-induced stx mRNA expression and Stx=20
> synthesis, without altering EHEC viability. The synthesis of stx=20
> phage is also reduced by NO. This inhibitory effect apparently=20
> occurs through the NO-mediated sensitization of EHEC because=20
> mutation of the NO sensor nitrite-sensitive repressor results in=20
> loss of NO inhibiting activity on stx expression. Thus our findings=20
> identify NO as an inhibitor of stx expressing-phage propagation and=20
> Stx release and thus as a potential protective factor limiting the=20
> development of hemolytic syndromes.
>
Sukie (not a vet)
Recommended ferret health links:
http://pets.groups.yahoo.com/group/ferrethealth/
http://ferrethealth.org/archive/
http://www.afip.org/ferrets/index.html
http://www.miamiferret.org/fhc/
http://www.ferretcongress.org/
http://www.trifl.org/index.shtml
http://homepage.mac.com/sukie/sukiesferretlinks.html
Yahoo! Groups Links
<*> To visit your group on the web, go to:
http://groups.yahoo.com/group/ferrethealth/
<*> Your email settings:
Individual Email | Traditional
<*> To change settings online go to:
http://groups.yahoo.com/group/ferrethealth/join
(Yahoo! ID required)
<*> To change settings via email:
mailto:ferrethealth-digest@yahoogroups.com
mailto:ferrethealth-fullfeatured@yahoogroups.com
<*> To unsubscribe from this group, send an email to:
ferrethealth-unsubscribe@yahoogroups.com
<*> Your use of Yahoo! Groups is subject to:
http://docs.yahoo.com/info/terms/