From:
ferrethealer@aol.com
Date: 2008-02-16 18:19:04 UTC
Subject: ADV testing Re: [ferrethealth] Digest Number 1060
To: ferrethealth@yahoogroups.com
In a message dated 02/16/08 5:23:51 AM Eastern Standard Time,
ferrethealth@yahoogroups.com writes:
1. CIEP is no longer being done by United Vaccines.
Question: Is the "CEP" done by the Blue Cross Animal
Hospital essentially the same test as the CIEP that was done by United?
**Yes, it is, and I have been very happy with them. I've sent them over
fifty samples so far, turnaround is rapid, I've had no issues.**
CIEP
detects the presence of antibodies - not the virus
and therefore cannot
tell if animal is viremic, a carrier,
or has eliminated the virus - just
detects exposure
2. IFA is no longer being done .
3. Avecon has an Ab - Elisa test - only detects actively replicating
ADV and ONLY ADV antibodies. (their claim..?truth/ADV and O
**Seems to be a fairly accurate claim. My concern with this test is that if
the disase is not actively replicating at the time the test is done, but
begins to replicate the next week, the test will be negative. I'm also less
enamored of the test on blood than on saliva, as the salivary glands seem to be a
primary site of virus replication.**
4.
Avecon has a QuickCheck Ab Test -- detects antibodies -
Avecon site
states comparable sensitivity and specificity to the Elisa
Question1: Does the Quickcheck test detect antibody without regard for active
replication?
Question
2: The Elisa info claims the test only binds to ADV antibodies,
but the
quickcheck info doesn't claim this - does this mean the quickcheck
doesn't bind only ADV antibodies but others as well?
(If so, how then is sensitivity and specificity comparable between the two?)
**Personally, I do not use the quickchek at all, although when it first came
out, I did. I've seen false positives and false negatives on it, when
compared with more accurate tests such as histopathology.**
5. University of Georgia
is developing a different Elisa test - more sensitive than CIEP
which
detects non-replicating ADV (and a shorter- 4 hour time turn-around )
-
not yet commercially available.
**I'm waiting and waiting for this one!**
6. DNA in situ -
for post or biopsy - is this commercially available? where?
**University of Georgia has done this in the past, pretty sure it's still
available, but haven't done it in a while.**
7. PCR at the University of Georgia - is this commercially available?
If so..any website? (can't find one)..
**Not sure if it's available at this time.**
Dr. Ruth
*****************************************
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