From:
Sukie Crandall
Date: 2008-07-06 02:00:00 UTC
Subject: [ferrethealth] 2 new ferret abstracts
To: fhl <ferrethealth@yahoogroups.com>
There were problems in the past when ferrets were not first checked
for ECE not were cats checked for FIP, both coronaviruses and then
very general tests were done. Surprise! They had intestinal
symptoms, rather than SARS symptoms. Gee. *IF* I read the below
abstract right the study abstract at
http://www.vetpathology.org/cgi/content/full/45/4/551
shows more careful work, but I am not a pathologist and could be
incorrect.
BEGIN QUOTE
Vet Pathol. 2008 Jul;45(4):551-62.
Pathology of Experimental SARS Coronavirus Infection in Cats and
Ferrets.
van den Brand JM, Haagmans BL, Leijten L, van Riel D, Martina BE,
Osterhaus AD,Kuiken T.
Department of Virology, Erasmus Medical Center, Dr. Molewaterplein 50,
3015 GE Rotterdam, (The Netherlands).
The pathology of severe acute respiratory syndrome-coronavirus (SARS-
CoV) infection in cats and ferrets is poorly described, and the
distribution of angiotensin-converting enzyme 2 (ACE2), a receptor for
SARS-CoV, in the respiratory tracts of these species is unknown. We
observed SARS-CoV antigen expression and lesions in the respiratory
tracts of 4 cats and 4 ferrets at 4 days postinoculation and ACE2
expression in the respiratory tracts of 3 cats and 3 ferrets without
infection. All infected cats and ferrets had diffuse alveolar damage
associated with SARS-CoV antigen expression. A novel SARS-CoV-
associated lesion was tracheo-bronchoadenitis in cats. SARS-CoV
antigen expression occurred mainly in type I and II pneumocytes and
serous cells of tracheo-bronchial submucosal glands of cats and in
type II pneumocytes of ferrets. ACE2 expression occurred mainly in
type I and II pneumocytes, tracheo-bronchial goblet cells, serous
epithelial cells of tracheo-bronchial submucosal glands in cats, and
type II pneumocytes and serous epithelial cells of tracheo-bronchial
submucosal glands in ferrets. In conclusion, the pathology of SARS-CoV
infection in cats and ferrets resembles that in humans except that
syncytia and hyaline membranes were not observed. The identification
of tracheo-bronchoadenitis in cats has potential implications for SARS
pathogenesis and SARS-CoV excretion. Finally, these results show the
importance of ACE2 expression for SARS-CoV infection in vivo: whereas
ACE2 expression in type I and II pneumocytes in cats corresponded to
SARS-CoV antigen expression in both cell types, expression of both
ACE2 and SARS-CoV antigen in ferrets was limited mainly to type II
pneumocytes.
PMID: 18587105
END QUOTE
A second new abstract appears less useful for veterinary knowledge
(but I could be wrong, of course):
BEGIN QUOTE
Neurosci Lett. 2008 Jun 11. [Epub ahead of print] Links
P2X(3) expression is not altered by lingual nerve injury.
Biggs JE, Yates JM, Loescher AR, Clayton NM, Robinson PP, Boissonade FM.
Department of Oral & Maxillofacial Surgery, School of Clinical
Dentistry, University of Sheffield, UK; Department of Pharmacology,
University of Alberta, 9.75 Medical Sciences Building, Edmonton,
Alberta, Canada T6G 2H7.
We have investigated a possible role for the ATP receptor subunit
P2X(3), in the development of neuropathic pain following injury to a
peripheral branch of the trigeminal nerve. In nine anaesthetised adult
ferrets the left lingual nerve was sectioned and recovery permitted
for 3 days, 3 weeks or 3 months (3 ferrets per group). A retrograde
tracer, fluorogold, was applied to the nerve to allow identification
of cell bodies in the trigeminal ganglion with axons in the injured
nerve. Indirect immunofluorescence for P2X(3) and image analysis was
used to quantify the percentage area of staining at the site of
injury. Additionally, the proportion of fluorogold-positive cells that
expressed P2X(3) was determined and compared with expression in non-
fluorogold containing cells in another part of the ganglion.
Comparisons were made with results from control animals that only
received the tracer injection. After lingual nerve injury there was no
significant change in P2X(3) expression at the site of nerve injury or
within cell bodies linked to either injured (lingual) or uninjured
(ophthalmic) axons, at any of the time periods investigated. Overall,
this study suggests that P2X(3) expression at these sites is not
involved in the development of neuropathic pain following lingual
nerve injury.
PMID: 18597934
END QUOTE
Sukie (not a vet)
Recommended ferret health links:
http://pets.groups.yahoo.com/group/ferrethealth/
http://ferrethealth.org/archive/
http://www.afip.org/ferrets/index.html
http://www.miamiferret.org/fhc/
http://www.ferretcongress.org/
http://www.trifl.org/index.shtml
http://homepage.mac.com/sukie/sukiesferretlinks.html
[Non-text portions of this message have been removed]
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