From:
Sukie Crandall
Date: 2009-01-22 03:49:46 UTC
Subject: [ferrethealth] abstract
To: fhl <ferrethealth@yahoogroups.com>
http://www.ncbi.nlm.nih.gov/pubmed/19144625
> Vet Pathol. 2009 Jan 13. [Epub ahead of print]
> Alcian Blue and Pyronine Y histochemical stains permit assessment of
> multiple parameters in pulmonary disease models.
>
> Meyerholz D, Rodgers J, Castilow E, Varga S.
> UI Carver College of Medicine, 145 MRC, Iowa City, Iowa, 52242,
> United States of America.
> Utilization of a combined Alcian Blue and Pyronine Y histochemical
> method for the assessment of multiple parameters in the respiratory
> tract of various species is described. Acidic mucins were deep blue
> (sialylated mucins), red (sulfated mucins), or variably purple
> (mixture of sialylated/sulfated mucins), and differential mucus
> production was readily detected in a murine respiratory syncytial
> virus vaccine model of pulmonary inflammation. Elastic fibers
> stained red in the walls of pulmonary arteries, connecting airways,
> alveolar septa, and subpleural interstitium. Mast cells had red to
> red-purple granular cytoplasmic staining. Nuclei were ubiquitously
> counterstained pale blue. Representative staining was detected in
> tissues from multiple species including inbred mice, rats, ferrets,
> cats, dogs, sheep, and pigs. The fluorescent property of the stained
> tissues offers additional modalities with which to analyze tissue
> sections. This histochemical technique detects multiple critical
> parameters in routine paraffin sections of lung tissue, reduces the
> need for repeated serial sectioning and staining, and is cost-
> effective and simple to perform.
> PMID: 19144625
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