From:
Sukie Crandall
Date: 2009-01-24 02:22:52 UTC
Subject: [ferrethealth] abstract: cytokines
To: fhl <ferrethealth@yahoogroups.com>
http://www.ncbi.nlm.nih.gov/pubmed/19157571
BEGIN QUOTE
Vet Immunol Immunopathol. 2008 Dec 9. [Epub ahead of print]
Quantitative analysis of inflammatory cytokines expression in
peripheral blood mononuclear cells of the ferret (Mustela putorius
furo) using real-time PCR.
Nakata M, Itou T, Sakai T.
Nihon University Veterinary Research Center, 1866 Kameino, Fujisawa,
Kanagawa 252-8510, Japan.
This study describes the expression pattern of cytokines, interferon-
gamma (IFN-gamma), interleukin-6 (IL-6), tumor necrosis factor-alpha
(TNF-alpha) and IL-10, produced by LPS stimulation in peripheral blood
mononuclear cells (PBMCs) of the ferret (Mustela putorius furo). Real-
time PCR was used with TaqMan probes, which were modified by dual-
labeled probes (TAMRA/FAM), quantitative analysis of cytokine mRNA
comparing the cytokine with the housekeeping gene, ferret GAPDH, as
the relative C(t) value. Expression peaks of IFN-gamma, TNF-alpha,
IL-6 mRNA occurred 2h after LPS stimulation, whereas the IL-10 peak
was 8h post-LPS. In the present study, peak cytokine expression was
detected within 8h, similar to several other mammalian studies. This
current study provides baseline information on inflammatory cytokines
of ferret PBMCs.
PMID: 19157571
END QUOTE
Sukie (not a vet)
Recommended ferret health links:
http://pets.groups.yahoo.com/group/ferrethealth/
http://ferrethealth.org/archive/
http://www.afip.org/ferrets/index.html
http://www.miamiferret.org/fhc/
http://www.ferretcongress.org/
http://www.trifl.org/index.shtml
http://homepage.mac.com/sukie/sukiesferretlinks.html
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