From: Sukie Crandall
Date: 2009-09-10 19:20:41 UTC
Subject: [ferrethealth] published: rotavirus
To: fhl <email@example.com>
The article for this is now available:
> Vet Pathol. 2009 Sep;46(5):985-91. Epub 2009 May 9.
> Detection of group C rotavirus in juvenile ferrets (Mustela putorius
> furo) with diarrhea by reverse transcription polymerase chain
> reaction: sequencing and analysis of the complete coding region of
> the VP6 gene.
> Wise AG, Smedley RC, Kiupel M, Maes RK.
> Diagnostic Center for Population and Animal Health, Michigan State
> University, Lansing, MI 48910, USA.
> Nine juvenile ferrets (Mustela putorius furo) with a history of
> diarrhea were severely dehydrated and had distended abdomens and
> thin-walled small intestines that contained gas and fluid.
> Histologically, small intestines exhibited acute superficial
> atrophic enteritis. Transmission electron microscopy of the small
> intestine showed rotavirus-like particles within apical vacuoles.
> Reverse transcription polymerase chain reaction (RT-PCR) was
> negative for group A rotavirus. A group C rotavirus-specific RT-PCR
> assay was developed using consensus primers designed from the
> alignment of VP6 gene sequences of porcine, bovine, and human
> strains. A 182-bp product of the VP6 gene was sequenced and showed
> significant similarity to group C rotavirus VP6 sequences. This
> strain was designated "Ferret Rota C-MSU." The entire coding
> sequence of VP6 was determined and compared with other rotaviruses.
> Ferret Rota C-MSU virus was found to be most closely related to
> Shintoku group C rotavirus. This is the first definitive
> identification of a group C rotavirus in ferrets, based upon RT-PCR,
> sequencing, and genetic analysis.
> PMID: 19429992 [PubMed - in process]
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Sukie (not a vet)
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