Message Number: FHL10704 | New FHL Archives Search
From: Sukie Crandall
Date: 2010-01-09 05:58:16 UTC
Subject: [ferrethealth] abstract: giardia
To: fhl <ferrethealth@yahoogroups.com>


> Parasitol Res. 2010 Jan 7. [Epub ahead of print]
> Molecular characterization of Giardia duodenalis isolates from
> domestic ferrets.
> Abe N, Tanoue T, Noguchi E, Ohta G, Sakai H.
> Department of Microbiology, Osaka City Institute of Public Health
> and Environmental Sciences, Tennoji-ku, Osaka, 543-0026, Japan
> Giardia duodenalis is a pathogen that has been found in a variety of
> mammals, including humans, and consists of host-specific
> (assemblages C, D, E, F, and G) and zoonotic (assemblages A, B)
> genetic groups. Ferrets are popular pets, and they, like humans, are
> hosts to this pathogen. The genetic characteristics of the Giardia
> population in ferrets are unclear because only one ferret isolate
> has been genotyped. To develop a more complete picture of the
> genetic characteristics of the Giardia population in domestic
> ferrets two additional Giardia isolates, recovered independently
> from two ferrets suffering from intestinal or hepatic giardiasis,
> were analyzed genetically. The sequences of both isolates at three
> loci, small subunit ribosomal RNA (SSUrDNA, 292 bp), ss-giardin (734
> bp), and glutamate dehydrogenase (GDH, 713 bp) were identical to
> each other, but the sequences of triosephosphate isomerase locus
> (TPI, 512 bp) had five substitutions between isolates. Sequence
> homology and phylogenetic analyses at four loci identified both
> isolates as members of the assemblage A. Moreover, the sequences of
> SSUrDNA, ss-giardin, and GDH from both isolates were identical to
> those of the previous ferret isolate genotyped as assemblage A
> within the regions of overlap. The result obtained in the present
> study indicates that at least two genetically different types in
> assemblage A exist in domestic ferrets. In addition, there have been
> no reported human and animal isolates with the same sequence as
> those from ferret isolates at all four loci examined, suggesting
> that the present ferret isolates might be host-specific.
> PMID: 20054561

Sukie (not a vet)

Recommended ferret health links:
http://pets.groups.yahoo.com/group/ferrethealth/
http://ferrethealth.org/archive/
http://www.afip.org/ferrets/index.html
http://www.miamiferret.org/
http://www.ferrethealth.msu.edu/
http://www.ferretcongress.org/
http://www.trifl.org/index.shtml
http://homepage.mac.com/sukie/sukiesferretlinks.html
all ferret topics:
http://listserv.ferretmailinglist.org/archives/ferret-search.html

"All hail the procrastinators for they shall rule the world tomorrow."
(2010, Steve Crandall)






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