From:
Sukie Crandall
Date: 2010-03-12 18:24:45 UTC
Subject: [ferrethealth] Helicobacter mustelae genome study
To: fhl <ferrethealth@yahoogroups.com>
. BMC Genomics. 2010 Mar 10;11(1):164. [Epub ahead of print]
Comparative genomics and proteomics of Helicobacter mustelae, an
ulcerogenic and carcinogenic gastric pathogen.
O'Toole PW, Snelling WJ, Canchaya C, Forde BM, Hardie KR, Josenhans
C, Graham RL, McMullan G, Parkhill J, Belda E, Bentley SD.
ABSTRACT:
BACKGROUND: Helicobacter mustelae causes gastritis, ulcers and gastric
cancer in ferrets and other mustelids. H. mustelae remains the only
helicobacter other than H. pylori that causes gastric ulceration and
cancer in its natural host. To improve understanding of H. mustelae
pathogenesis, and the ulcerogenic and carcinogenic potential of
helicobacters in general, we sequenced the H. mustelae genome, and
identified 425 expressed proteins in the envelope and cytosolic
proteome. RESULTS: The H. mustelae genome lacks orthologs of major H.
pylori virulence factors including CagA, VacA, BabA, SabA and OipA.
However, it encodes ten autotransporter surface proteins, seven of
which were detected in the expressed proteome, and which, except for
the Hsr protein, are of unknown function. There are 26 putative outer
membrane proteins in H. mustelae, some of which are most similar to
the Hof proteins of H. pylori. Although homologs of putative virulence
determinants of H. pylori (NapA, plasminogen adhesin, collagenase) and
Campylobacter jejuni (CiaB, Peb4a) are present in the H. mustelae
genome, it also includes a distinct complement of virulence-related
genes including a haemagglutinin/haemolysin protein, and a glycosyl
transferase for producing blood group A/B on its lipopolysaccharide.
The most highly expressed 264 proteins in the cytosolic proteome
included many corresponding proteins from H. pylori, but the rank
profile in H. mustelae was distinctive. Of 27 genes shown to be
essential for H. pylori colonization of the gerbil, all but three had
orthologs in H. mustelae, identifying a shared set of core proteins
for gastric persistence. CONCLUSIONS: The determination of the genome
sequence and expressed proteome of the ulcerogenic species H mustelae
provides a comparative model for H. pylori to investigate bacterial
gastric carcinogenesis in mammals, and to suggest ways whereby cag
minus H. pylori strains might cause ulceration and cancer. The genome
sequence was deposited in EMBL/GenBank/DDBJ under accession number
FN555004. PMID: 20219135 [PubMed - as supplied by publisher]
Sukie (not a vet)
Recommended ferret health links:
http://pets.groups.yahoo.com/group/ferrethealth/
http://ferrethealth.org/archive/
http://www.afip.org/ferrets/index.html
http://www.miamiferret.org/
http://www.ferrethealth.msu.edu/
http://www.ferretcongress.org/
http://www.trifl.org/index.shtml
http://homepage.mac.com/sukie/sukiesferretlinks.html
all ferret topics:
http://listserv.ferretmailinglist.org/archives/ferret-search.html
HAPPY: http://www.6footsix.com/my_weblog/2010/01/high-fives-for-happiness.html
"All hail the procrastinators for they shall rule the world tomorrow."
(2010, Steve Crandall)
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