From:
Sukie Crandall
Date: 2010-07-22 19:13:12 UTC
Subject: [ferrethealth] abstract
To: fhl <ferrethealth@yahoogroups.com>
There are other recent ones which are less applicable for the FHL on
PubMed, too.
This is the sort of info which may ultimately result in better medical
approaches.
Infect Immun. 2010 Jul 19. [Epub ahead of print]
An ABC transporter and a TonB ortholog contribute to Helicobacter
mustelae nickel and cobalt acquisition.
Stoof J, Kuipers EJ, Klaver G, van Vliet AH.
Department of Gastroenterology and Hepatology, Erasmus MC - University
Medical Center, 's Gravendijkwal 230, 3015 CE Rotterdam, The
Netherlands; Department of Internal Medicine, Erasmus MC - University
Medical Center, 's Gravendijkwal 230, 3015 CE Rotterdam, The
Netherlands; TNO, P.O. Box 80.015, 3508 TA Utrecht, The Netherlands;
Institute of Food Research, Foodborne Bacterial Pathogens Programme,
Colney Lane, Norwich NR4 7UA, United Kingdom.
Abstract
The genomes of Helicobacter species colonizing the mammalian gastric
mucosa (like Helicobacter pylori) contain a large number of genes
annotated as iron acquisition genes, but only few nickel acquisition
genes, which contrasts with the central position of nickel in urease-
mediated acid resistance of these gastric pathogens. In this study we
have investigated the predicted iron and nickel acquisition systems of
the ferret pathogen Helicobacter mustelae. Expression of the outer
membrane protein-encoding frpB2 gene was iron- and Fur-repressed,
whereas expression of the ABC transporter genes fecD and ceuE was
iron- and Fur-independent. Inactivation of the two tonB genes showed
that TonB1 is required for heme utilization, whereas absence of TonB2
only marginally affected iron-dependent growth but led to reduced
cellular nickel content and urease activity. Inactivation of the fecD
and ceuE ABC-transporter genes did not affect iron levels, but
resulted in a significant reduced urease activity and cellular nickel
content. Surprisingly, inactivation of the nixA nickel transporter
gene only affected cellular nickel content and urease activity when
combined with inactivation of other nickel acquisition genes, like
fecD or ceuE. The FecDE ABC transporter is not specific for nickel,
since a fecD mutant also showed reduced cellular cobalt levels and
increased cobalt resistance. We conclude that the H. mustelae fecDE
and ceuE genes encode an ABC transporter involved in nickel and cobalt
acquisition, which works independently of the nickel transporter NixA,
while TonB2 is primarily required for nickel acquisition, with TonB1
being required for heme utilization.
PMID: 20643857 [PubMed - as supplied by publisher]
Sukie (not a vet)
Recommended ferret health links:
http://pets.groups.yahoo.com/group/ferrethealth/
http://ferrethealth.org/archive/
http://www.afip.org/ferrets/index.html
http://www.miamiferret.org/
http://www.ferrethealth.msu.edu/
http://www.ferretcongress.org/
http://www.trifl.org/index.shtml
http://homepage.mac.com/sukie/sukiesferretlinks.html
all ferret topics:
http://listserv.ferretmailinglist.org/archives/ferret-search.html
"All hail the procrastinators for they shall rule the world tomorrow."
(2010, Steve Crandall)
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