From:
Sukie Crandall
Date: 2010-08-05 19:49:56 UTC
Subject: [ferrethealth] abstract
To: fhl <ferrethealth@yahoogroups.com>
There are others which are less applicable for the FHL's focus.
http://www.ncbi.nlm.nih.gov/pubmed/20682846
> Physiol Genomics. 2010 Aug 3. [Epub ahead of print]
> Expression and Distribution of Voltage Gated Ion Channels in Ferret
> Sinoatrial Node.
> Brahmajothi MV, Morales MJ, Campbell DL, Steenbergen C, Strauss HC.
> Duke University Medical Center.
> Spontaneous diastolic depolarization in the sinoatrial (SA) node
> enables it to serve as pacemaker of the heart. The variable cell
> morphology within the SA node predicts that ion channel expression
> would be heterogeneous and different from that in the atrium. To
> evaluate ion channel heterogeneity within the SA node, we used
> fluorescent in situ hybridization to examine ion channel expression
> in the ferret SA node region and atrial appendage. SA nodal cells
> were distinguished from surrounding cardiac myocytes by expression
> of the slow (SA node) and cardiac (surrounding tissue) forms of
> troponin I. Nerve cells in the sections were identified by detection
> of GAP-43 and cytoskeletal middle neurofilament. Transcript
> expression was characterized for the 4 hyperpolarization-activated
> cation channels, 6 voltage-gated Na(+) channels, 3 voltage-gated
> Ca(2+) channels, 24 voltage-gated K(+) channel alpha subunits and 3
> ancillary subunits. To ensure transcript expression was
> representative of protein expression, immunofluorescence was used to
> verify localization patterns of voltage-dependent K(+) channels. Co-
> localizations were performed to observe any preferential patterns.
> There were some overlapping and non-overlapping binding patterns
> observed. Measurement of different cation channel transcripts showed
> heterogeneous expression with many different patterns of expression,
> attesting to the complexity of electrical activity in the SA node.
> This study provides insight in to the possible role ion channel
> heterogeneity plays in SA node pacemaker activity. PMID: 20682846
> [PubMed - as supplied by publisher]
Sukie (not a vet)
Recommended ferret health links:
http://pets.groups.yahoo.com/group/ferrethealth/
http://ferrethealth.org/archive/
http://www.afip.org/ferrets/index.html
http://www.miamiferret.org/
http://www.ferrethealth.msu.edu/
http://www.ferretcongress.org/
http://www.trifl.org/index.shtml
http://homepage.mac.com/sukie/sukiesferretlinks.html
all ferret topics:
http://listserv.ferretmailinglist.org/archives/ferret-search.html
"All hail the procrastinators for they shall rule the world tomorrow."
(2010, Steve Crandall)
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